How do i add a physikal scaling to an axis (f.a. centimeters) and save these informations together with the image into a .mat file?

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I want to calculate an average image from 50 single images, show this one in my GUI together with two real axis and later on save the image together with the axes into one matrix as .mat file.
Does matlab offer any kind of operation to realize this or do I have to collect all my information together, put it in an array and save this on as usual?
thanks, Marc

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