Cell Image Processing Segmentation/Cell Counting Problem

Hi! I am currently looking at cancer squamous carcinoma cells. I am attempting to process and segment the cells so I will be able to count them, but it is difficult for me to segment them because the cells are overlapping and do not have well-defined borders. I have tried turning the picture to black & white using a threshold (im2bw(X, graythresh(X)), but it results in poorly segmented cells that are hardly defined. I would also like to be able to automatically count the cells. Any tips on how to tackle this problem with MATLAB? Below I have attached the original cell image, and my attempt to process it for automated counting. Any help would be greatly appreciated!

[KALYAN ACHARJYA]

Image Analyst Sir

[Emma Wong]

Hi Sir, do you have any suggestions for this problem?

[Rishab Agarwal]

Any leads here? If yes, please help me out to go through similar situation.

Answer 1

[Sarah Salvino]

I've a similar problem, and I'm still searching for a solution. Dou you discover how to process this kind of image? Thanks