How to convert pixel coordinate system to image coordinate system

x=(y'-ne/2.0)*y_pix_size
y-(nr/2.0-x')*x_pix_size

where

ne= number of columns
nr= number of rows
x_pix_size= pixel size along the row direction
y_pix_size= pixel size along the column direction

HELP ME PLEASE .....

Answer 1

[KSSV]

Let I be your image.

[m,n,p] = size(I) ; 
r = 1:n ;
c = 1:m ;
[X,Y] = meshgrid(r,c) ; 

[Keeyong Kim]

then how to do next process ...?

Answer 2

[Image Analyst]

Compute the spatial calibration factor by dividing your field of view in microns by the number of pixels across:

[rows, columns, numberOfColorChannels] = size(yourImage);
micronsPerPixel = fieldOfVewInMicrons / columns;
xInMicrons = xInPixels * micronsPerPixel;
yInMicrons = yInPixels * micronsPerPixel;

and for any distances between points that you compute, or areas:

distanceInMicrons = distanceInPixels * micronsPerPixel;
areaInSquareMicrons = areaInPixels * micronsPerPixel ^ 2;

Or you could just compute the distances and areas from the micron version of x and y directly.