Particle Tracking

I am doing some research and need to track the displacement of nanoparticles in a video that I captured. I have started to write the code; however, I am stuck.

I need to find a way to label each particle and determine in the next frame where that particle moved to. I have calculated the centroid of each particle and written them to a table. I am not sure how to compare these centroid particles and track them across 50 frames. Particles tend to come and go as they go in and out of the focal plane.

Thanks.

Code:

clear all; 
mov = aviread('50Mol_Simul.avi'); %Convert video to images
for m =1:length(mov)
 [im,map] = frame2im(mov(m));
 imwrite(im, ['im' num2str(m) '.jpg'],'jpg');
end 
mov = mmreader('50Mol_Simul.avi');
implay('50Mol_Simul.avi');
nFrames = mov.NumberOfFrames
Width = mov.Width
Height = mov.Height

for k = 1 : nFrames
  singleFrame = read(mov, k);
  level = graythresh(singleFrame);
  bw = im2bw(singleFrame, level);         
%     figure;
%     imshow(bw);
  bw = bwareaopen(bw, 3);
  L = bwlabel (bw,8);
  stats = regionprops(bw,'Centroid');
  V = cat(1,stats.Centroid);
  temp.ctrd = V;
  temp.index = zeros(size(V,1),1);
  a(k) = temp;
end

I am not sure how to attach the video file. If some one tells, I can upload that as well.

Answer 1

[Jianan]

I am not good at coding, but I used the codes below for my experimental images. It works very well. Hope it will be helpful for you too.

http://physics.georgetown.edu/matlab/tutorial.html

[Prasad Kalane]

Nice information, Thanks Jianan

[TripleCaffeine]

link is now dead

[Shweta Narayan]

Here's a working link:

http://site.physics.georgetown.edu/matlab/code.html

[Arash Beyrami]

Could you please explain more that how it works?