PCRefficiency

Set the Efficiency of a RT-PCR to use in the relative quantification of transcripts.
2.1K Downloads
Updated 4 Apr 2018

Reverse transcription(RT) followed by PCR is a powerful tool for the detection and quantification of mRNA. It is the most sensitive method for the detection and quantification of gene expression levels, in particular for low abundance mRNA. The relative quantification is based on the expression ratio of a target gene versus a reference gene. Some mathematical models have already been developed to calculate the relative expression ratios, with or without efficiency correction. Normally the PCR efficiency is set at 2 (the max possible value) for the reference and target gene, but a difference in PCR efficiency of 0.03 between the target and reference gene, the falsely calculated difference in expression ratio is 46% in case of Et<Er and 209% in the case of Et>Er. The difference will increase dramatically by higher efficiency differences: i.e. DE=0.05 (27% and 338%) and DE=0.1 (7.2% and 1083%) This function computes the efficiency of PCR reaction and is based on MYREGR function. If it is not present on the computer, pcreff will try to download it from FEX
Created by Giuseppe Cardillo
giuseppe.cardillo-edta@poste.it

To cite this file, this would be an appropriate format: Cardillo G. (2008) PCREfficiency: set the Efficiency of a RT-PCR to use in the relative quantification of transcripts. http://www.mathworks.com/matlabcentral/fileexchange/20887

Cite As

Giuseppe Cardillo (2024). PCRefficiency (https://github.com/dnafinder/pcreff), GitHub. Retrieved .

MATLAB Release Compatibility
Created with R2014b
Compatible with any release
Platform Compatibility
Windows macOS Linux

Community Treasure Hunt

Find the treasures in MATLAB Central and discover how the community can help you!

Start Hunting!

Versions that use the GitHub default branch cannot be downloaded

Version Published Release Notes
2.0.0.0

inputparser; table implementation; github link

1.5.0.0

Switched from natural logarithm to decimal logarithm to uniform the output to Roche and Applied Biosystems outputs

1.4.0.0

This routine uses MYREGR function. If it is not present on the computer, PCREff will try to download it from FEX.

1.3.0.0

Changes in description

1.2.0.0

Changes ih help section

1.0.0.0

To view or report issues in this GitHub add-on, visit the GitHub Repository.
To view or report issues in this GitHub add-on, visit the GitHub Repository.