Measuring average distance between two lines in an OCT Image

Greetings,

I am working on a project and my aim is to calculate average thickness of different retinal layers. Is there anyway I could calculate the averaged, min and max distance between these two lines using MATLAB?

Thanks

Answer 1

[Image Analyst]

Just scan across the thresholded image finding the first and last rows where you have a pixel. Then you might have to get rid of outliers, maybe with a median filter. Then compute the area and divide by the width. Something like (untested):

[rows, columns, numberOfColorChannels] = size(grayImage);
mask = grayImage > someThreshold.
topRows = zeros(1, columns);
bottomRows = zeros(1, columns);
for col = 1 : columns
    t = find(mask(:, col), 1, 'first');
    if ~isempty(t)
        topRows(col) = t;
        bottomRows(col) = find(mask(:, col), 1, 'last');
    end
end
x = 1 : columns;
hold on;
plot(x, topRows, 'r-', 'LineWidth', 2);
plot(x, bottomRows, 'r-', 'LineWidth', 2);
% Get heights as function of column.
heights = bottomRows - topRows;
% That is the area of each column. 
% Divide by the number of columns to get the mean height.
meanHeight = sum(heights) / columns;

If you still need help, attach the original image (without any lines or annotations).

[Mohammad Mehdi]

Thank you very much for the promot reply,

Actually I am trying to get the measurment of different layers of retina:

and I am quite new with matlab, so it would be great if you could help me, please.

I have attached the raw image, too.