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How to perform image segmentation
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I want to manually segment a small object(eg: a feature similar to cell nucleus) which is located within the original image. Can anyone kindly tell me what is the cause to obtain the boundary of the segmented image larger than the real image? (though i drew the boundary along the cell nucleus, segmented image still gives larger segmentation.
Thank you.
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Image Analyst
il 29 Apr 2015
See my Image Segmentation Tutorial : http://www.mathworks.com/matlabcentral/fileexchange/25157-image-segmentation-tutorial
![](https://www.mathworks.com/matlabcentral/images/broken_image.png)
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Image Analyst
il 12 Mag 2015
Chathu, that's what my Image Segmentation Tutorial does. http://www.mathworks.com/matlabcentral/fileexchange/25157-image-segmentation-tutorial Just tell it to get the dark things instead of the bright things. It will crop them out into separate subimages. Then you can just save the subimages with imwrite().
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Thomas Koelen
il 29 Apr 2015
If I understand you correctly, you want to extract only the nucleus, and not the background, this is impssible because you can't make an image that's not a rectangle. You can however make the backround white.
Chathu
il 12 Mag 2015
2 Commenti
Thomas Koelen
il 12 Mag 2015
Since the part you are trying to get is way darker than the other parts I suggest you can do something like this:
(im using a paint image because you didn't supply your own image wihout the arrow)
![](https://www.mathworks.com/matlabcentral/answers/uploaded_files/183249/image.png)
IM=imread('chathu.png');
figure
IM(IM>30)=255;
bw1=im2bw(IM);
imshow(bw1);
IM2=medfilt2(bw1);
stats = regionprops(IM2,'Centroid');
which give soyu the center of the "blob":
stats =
Centroid: [134.3417 148.9362]
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