What is the best method for analyzing bacteria?
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I'm trying to track the position of bacteria over generations and have attempted two methods of doing so. The first involves large amounts of adjusting image processing parameters such as strel which has been successful but takes along time. I have also been attempting to use statistical methods such as a using histograms to get rid of the background however I am having some issues with locating the correct threshold to use in im2bw. If anyone could give me some pointers I would be very grateful! One of the images I am using is attached
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Image Analyst
il 20 Lug 2016
I don't know why determining parameters takes such a long time. Anyway, why can't you just threshold and find the centroid?
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