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I have two images taken by projecting laser light onto a agar plate. We have taken images at starting point and after an interval of 20 minutes, where we cultured bacteria on it. Then we applied imsubtract(a,b), (b,a) both giving different answers ?
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a=imread('im5.jpg');
b=imread('im7.jpg');
c=imsubtract(a,b):
imshow(c)
d=imsubtract(b,a);
imshow(d)
after that in the output side we got greenish and red color image. Here we used a red color laser light. My question is, is that greenish color represents bacterial colony or anything else. Why the output is changing after interchanging these a,b to b,a in 'imsubtract' function? Following are the images: mat1 - for imsubtract(a,b) and mat2 - for imsubtract(b,a).
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KALYAN ACHARJYA
il 20 Dic 2018
Modificato: KALYAN ACHARJYA
il 20 Dic 2018
Yes, how you expect that both gives the same results?
A-B !=B-A (!=>Not Equal)
Note: Subtraction and division functions are Not Commutative.
In your case
imsubtract(a,b)=a-b
and
imsubtract(b,a)=b-a
Both are not same.
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