Method to determine the difference between 2 images

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I need suggestion on how to differenstiate the diffetence between this 2 images. The help will be much more appreciate =)
Better is with coding explanation.

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Image Analyst
Image Analyst il 10 Feb 2019
There are lots of things that are different between those images, like size, area, count, and shape of dark blobs. See my Image Segmentation Tutorial in my File Exchange

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