background of an image to 0
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I have a 2d image( from microscope of cells) and I am trying to quantify intensity of fluoroscence. However i did not take a background fluoro image and was wondering if there is any way to polyfit the image to bring the offset to 0.
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Luis J Gilarranz
il 23 Mag 2019
Depending on what the images represent you could normalize them (dividing all the values by the maximum fluorescence value recorded) and then apply the threshold I was mentioning. However, if you have a treatment that affect the maximum fluorescence values then you could not do this and you could not compare across treatments. It would help if you put more information.
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Luis J Gilarranz
il 22 Mag 2019
Testing different thresholds to see which one removes the background without removing any cell...
%Identify the pixels abobe the threshold
Mask = Image>Threshold;
%Set the background to zero
NewImage = Image.*Mask;
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