DICOM Alignment (slice by slice substraction)

Hello,

I have many DICOM serial images (210 slices each one) for the same core but in two different conditions. I need to subtract two files (Slice by slice) in the same location and compare them. Let's assume the image parameters like pixel spacing, slice thickness, etc. are the same. But the core may moved after each scan. How can I achieve the image alignment? in the end, I would like to find a 2D matrix (i, j) with the pixel by pixel substraction values between the two series of images.

Thank you!

[Rik]

Are there any object features you could automatically detect? If so, you can use that as landmarks to determine the required transformation.

[Madi khad]

No there is no object features that i can detect.I'am trying with imregtform, have any idea how to use it?

Thank you

Answer 1

[Selva Karna]

Set thresold on slides

Segment objecct and convert as volume

[Madi khad]

I'am trying with imregtform, have any idea how to use it?

thank you