Averaging brightness/fluorescent intensity in columns (y axis)
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Hello, I'm trying to find the average brightness of the columns (y direction) of an image and have these for each column along the x direction. Then I'd like to plot this in some sort of graph.
I've gotten some good information through this forum:
grayImage = rgb2gray(Image);
meanGrayLevels = mean(grayImage(:));
I'm a little confused about how to set up the indices for the mean. And how do I set this in a loop to continue along the x direction of the image until the end of the image? Any help here would be much appreciated.