How to analyze every single pixels intensity in a video clip?

I need help get each single pixel's intensity in a video clip (grayscale). For example, in a video clip containing 150x250 pixels and 1000 frames, I need to obtain the intensity one by one, either row-wise or column-wise.

[DGM]

The set of all pixels in a video is the video itself. If you have read the video, there's nothing else to "get". Since we don't know what you're actually trying to do, there's really not much else to add other than to say that doing it one pixel at a time is probably not appropriate -- but then again, that's just a guess.

[Hirithar]

In our bodies, various types of movements occur involuntarily simultaneously, some in regular frequencies and others irregularly. Consider, for example, an ultrasound clip of an artery: with each heartbeat, the artery expands and then returns to its normal state. This results in changes in the pixel intensity around the circumference of the artery over time. Similarly, adjacent tissues also undergo movements, leading to changes in pixel intensity in those areas. I am interested in plotting the intensity of each pixel frame by frame. Does this explanation clarify my question?

[Image Analyst]

If you have, say, a million pixels in your video frame, you can't do a million line plots. But you can plot the average color or brightness of one, or a few, regions over time. This is what my Answer below does. Did you even see it (scroll down) or try it? If not, why not?

[Hirithar]

I downloaded it, I'm a medical student. I'm trying to undestand what the code is, for sure I will let you know. Thanks for the support.

Answer 1

[Image Analyst]

See my attached demo where I find the mean R, G, and B mean of every frame in a video and plot them. Adapt the region to what you need, like a single row or column instead of the whole frame.

Answer 2

[Hirithar]

I came up with this code for pixel analysis. Could you please take a look?

[Image Analyst]

Since crop is a 3-D array, what is your intention here when you use only two indexes to reference it?

array_Single(row,column,end) = crop(row ,column);
array_Single(row,column,(1:end-1)) = array_Single(row,column,(2:end));
% array_Single(row,column,c) = crop(row,column);
%array_Single(1:end-1) = array_Single(2:end);

[Hirithar]

I'm just picking up the intensity value of a pixel by its coordinates (row, column) to plot a 3-second spectrum. In my case, the frame rate is 30 fps, which means I'm plotting the spectrum for 90 frames.

[Image Analyst]

I strongly suggest that you use all three indexes when referencing a 3-D array.