How to calculate diameter of object
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Ahmed Ismail
il 9 Ago 2016
Commentato: Ahmed Ismail
il 10 Ago 2016
![](https://www.mathworks.com/matlabcentral/answers/uploaded_files/155580/image.png)
I would like to calculate the diameter of each of the particles so to generate the diameter distribution of the entire image.
![](https://www.mathworks.com/matlabcentral/answers/uploaded_files/155581/image.png)
X=Image;
template = fspecial('LoG',[51,51],3); % produces a laplacian of Gaussian curve as a template mask use to compare all the intensities in the image
y = conv2(double(X),-template,'same'); % convolves the image with the template to find all the points which correspond to the template mask
FoVY = y(500:700,730:940);
Peaks = imregionalmax(FoVY); % finds all the local maxima
imagesc(Peaks)
LPeaks = bwlabel(Peaks);
R = regionprops(LPeaks);
FoVY = X(500:700,730:940);
imagesc(FoVY);
hold on;
for i = 1:length(R)
plot(R(i).Centroid(1),R(i).Centroid(2),'r.');
centroid = cat(1, R.Centroid);
end
no_of_collagen=length(centroid);
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Image Analyst
il 9 Ago 2016
You can call regionprops() and then ask for EquivDiameter. You might get batter, more comprehensive answers if you put as much effort into your question here on the Mathworks MATLAB Central as you did for some other forum.
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Image Analyst
il 10 Ago 2016
It would be difficult, even manually with imfreehand. I can't really tell where one starts and the other stops. Why isn't the count enough? If you know the total area, and the count, you can get the average area of the collagen.
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