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version (957 KB) by Rob Campbell
Measure a point spread function from a microscope


Updated 21 Apr 2018

GitHub view license on GitHub

This function measures the full-width-half-max of a microscope point spread function (PSF) along 4 different dimensions: both x and y at the z-plane with the brightest intensity, and also along z/x and z/y. The user simply needs to provide a z-stack containing a PSF (e.g. by imaging a sub-micron fluorescent bead) and also the number of microns per pixel along x/y and z.

Cite As

Rob Campbell (2019). measurePSF (, GitHub. Retrieved .

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MATLAB Release Compatibility
Created with R2015a
Compatible with any release
Platform Compatibility
Windows macOS Linux