SimBiology

Segui

Hello, I have two sets of PK data from two versions of a molecule which is designed to bind to a target with a given Kd. Each data set represents the PK of the molecule which binds to the target at the corresponding Kd value. I'm looking to perform a pooled fit of both data sets to five parameters of a model which describes the kinetics of the molecule - it's a standard TMDD model -, kdeg, kint, R0, kon, koff. The objective of the fit is to obtain two parameter sets corresponding to the two data sets, where the value of kdeg, kint, and R0 are the same in each fit, and the value of kon and koff can vary per data set; but with the constraint that the Kd value is equal to the corresponding known experimentally measured value. How can I do this using the Model Analyzer GUI?

SimBiology implementation of the Target-Mediated Drug Disposition (TMDD) model